by: Ellen Rose E. Torremoro, Sharon N. Nuñal, Jant Cres C. Caigoy
This study describes the development of an enriched culture media for growth, biomass and carotenoid production of the photosynthetic bacteria Rhodobacter sphaeroides PSB1 through supplementation of fish processing wastes in the Acetate Yeast Extract (AYE) medium. Proximate compositions of the fish processing wastes were determined prior to supplementation. Cultures were incubated and analyzed for growth, biomass and carotenoid production after 14 days. Results showed that addition of fish viscera yielded significantly higher (P<0.05) growth (2.75 x105 ml-1 cells), protein content (71.37 μg mL-1) and carotenoid production (22.11 mg L-1) than supplementation of fish frames and control which may be attributed to the high protein and lipid content of the viscera. Biomass production was however higher in cultures supplemented with fish frames (22.67 g L1) which showed higher ash content than the viscera. Findings of this study showed that fish processing wastes may be used as supplemental nutrient source to promote the growth, biomass, protein and carotenoid production of R. sphaeroides, an industrially-important photosynthetic bacterium.
Keywords: Photosynthetic bacteria, Rhodobacter sphaeroides, fish processing wastes, biomass, carotenoid